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The mice had free access to food and water and were used in experiments at 4, 14, and 21 months of age

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2.6 RNA extraction and sequencing RNA extraction was performed using the Qiagen RNeasy Micro Kit (Qiagen #74004) according to the Purification of Total RNA from Animal and Human Cells protocol provided by the manufacturer

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For the pellet fractions (P1 and P2), we added 500 and 125 L of reagent B (75% dichloromethane (Fisher Scientific, AA22917K2 HPLC-grade) and 25% methanol), respectively

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If an IV is significantly associated with SZ related diseases or phenotypes (e.g., depression, anxiety disorder, affective disorder), it is identified as a potential confounder and excluded

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Int J Obes (Lond) 2012;36:735743

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