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The mice had free access to food and water and were used in experiments at 4, 14, and 21 months of age

2.6 RNA extraction and sequencing RNA extraction was performed using the Qiagen RNeasy Micro Kit (Qiagen #74004) according to the Purification of Total RNA from Animal and Human Cells protocol provided by the manufacturer

For the pellet fractions (P1 and P2), we added 500 and 125 L of reagent B (75% dichloromethane (Fisher Scientific, AA22917K2 HPLC-grade) and 25% methanol), respectively

If an IV is significantly associated with SZ related diseases or phenotypes (e.g., depression, anxiety disorder, affective disorder), it is identified as a potential confounder and excluded

Int J Obes (Lond) 2012;36:735743
