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The second enzyme is glutathione synthase

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The National Institutes of Health reports that regular consumption of foods rich in glutathione, like cruciferous vegetables (e.g., broccoli), may help increase levels of this antioxidant in the body by up to 30%

27 A tightness function has been described in various epithelia for claudin-1, -3, -4, -5, -8, -11, -14, and -19 using knockout mouse models or a range of assays (cited in Krause et al