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Then cells were fixating by 4% paraformaldehyde, blocking by 1% bovine serum albumin (BSA), incubating with the primary antibody (Calreticulin Rabbit Monoclonal Antibody) at 4 C overnight, staining with the secondary antibody (Goat Anti-Rabbit IgG (Alexa Fluor@488)) for 1 h and DAPI for 10 min at room temperature

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Glutathione is naturally produced by the body, but levels can be depleted due to stress, poor diet, and environmental factors such as pollution

Figure 3 The identification of genetic disease modifiers comes with great challenges with a requirement for clinical annotations to inform on disease progression or severity with well-powered case-case studies rather than case-control studies to understand disease progression in human fibrotic diseases