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The gene expressions and/or activities related to liver injury, lipid metabolism and oxidation, such as ACC, PPAR-, CHOP and Caspase-3, were assessed by real time PCR and western blot

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PE-22-28 inhibits TREK-1 with an IC50 of 0.12 nM, as determined by electrophysiology in HEK293 cells expressing human TREK-1

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