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Indirect immunofluorescence assay was conducted by our previously described methods [29]

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Metabolites as global regulators: A new view of protein regulation: Systematic investigation of metabolite-protein interactions may help bridge the gap between genome-wide association studies and small molecule screening studies

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In the evaluation of thioflavin-S and GSK-3 distribution, due to the inconsistent selection of brain area for each slide, the distribution was presented as the ratio of fluorescent signal to total hippocampus area to show the objectiveness (as percentage (%))