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Tranexamic Acid for Upstream Melanin Production Inhibition: Tranexamic Acid blocks the signalling pathway that instructs melanin to be produced in response to UV and inflammatory stimuli, addressing pigmentation-driven dark circles at the production stage before melanin is synthesised

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Key Vocabulary Terms Term Definition Affinity tag Peptide or protein tag used to purify other proteins Ligand Ion, small molecule, peptide, or protein that binds to an affinity tag Reactive Group Chemical moiety used to bind ligands to agarose Spacer Linker between the agarose bead and reactive group / ligand Activated Agarose is activated when a reactive group and spacer are chemically added to it Affinity Purification Affinity purification is a common technique that leverages naturally occurring interactions to enable protein purification

Consistent with the altered EMT profile, the OVCAR5 CBPR cells demonstrated enhanced migration and reduced proliferation, glycolysis, and oxidative phosphorylation