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Vector pCR8::ncr-1::gfp (pFC63) or pCR8::ncr-2::gfp (pFC64) was assembled of partial sequences from strain FCZ210 ( ncr-1(cfu39[ncr-1::GFP]) ) or FCZ216 ( ncr-2(cfu45[ncr-2::GFP]) ) genome, partial sequences from pCR8::ncr-1 (pFC43) or pCR8::ncr-2 (pFC44), and digestion products of Xba Itreated pFC43 or Xho Itreated pFC44 using NovoRec Plus One-step PCR Cloning Kit

Primary concerns include increased injection site reactions from multiple subcutaneous injections, difficulty attributing adverse effects to the correct agent, additional cost without proven benefit, and potential quality variability in compounded lipotropic products