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Archives of Gerontology and Geriatrics 117:105274
Disulfide bonds were then reduced with tris(2-carboxyethyl)phosphine hydrochloride and proteins precipitated with 10% sulfosalicylic acid

Defective human spermatozoa is characterized by the presence of large amounts of DNA damage, which is largely oxidative in nature and closely associated with defects in spermiogenesis, which are further associated with reduced fertility, increased rates of miscarriage, and an enhanced risk of disease in the offspring

doi: 10.1016/j.pain.2005.03.029

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