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Purbia et al

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In addition, heparanase expression is high, principally in the glomerulus, 2448 h after LPS treatment, which leads to the glomerular loss of HS and glycosaminoglycans, and is associated with high blood urea nitrogen (BUN), high urinary albumin/creatinine ratio, and glomerular injury (93, 94)

The positive transformants were selected on YPD plates with addition of antibiotics geneticin (G418, 4 mg/mL) or hygromycin B (HygB, 1 mg/mL) and verified by DNA sequencing after the integrated gene fragments were amplified by PCR using the corresponding primers and the genomic DNA templates

K., Sitkovsky, M