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Immediately, his face and eyelids were burnt
Cells were washed twice with the FLICA kit wash buffer and then incubated with LIVE/DEAD Fixable AQUA Dead Cells Stain (Thermo Fisher, USA) for 15 min at RT, followed by extracellular staining in PBS + 1% BSA with the following fluorochrome-conjugated antibodies for monocyte phenotyping: anti-CD14 BV605 [Clone: M5E2], anti-CD16 PE-Cy7/BV711 [Clone: 3G8], anti-CD3 PE [Clone: HIT3a] and anti-CCR2 PerCP Cy5.5 [Clone: K036C2] are from BioLegend
Global Spine journal 7:609-616, 2017 PMID: 28989838 Nicholson, K.J., Millhouse, P.W., Pflug, E., Woods, B., Schroeder, G.D., Anderson, D.G., Hilibrand, A.S., Kepler, C.K., Kurd, M.F., Rihn, J.A., Vaccaro A.R.., Radcliff , C.: Cervical sagittal range of motion as a predictor of symptom severity in cervical spondylotic myelopathy
MoreiraJDKnorrLGanzellaMThomaziAPde SouzaCGde SouzaDGet al
The product of this process is primarily the hydroxy free radical, the most poisonous of all ROS
