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[DOI] [PMC free article] [PubMed] [Google Scholar] 28.Kadaja M, Keyes BE, Lin M, Pasolli HA, Genander M, Polak L, Stokes N, Zheng D, Fuchs E

Am J Physiol Regul Integr Comp Physiol 309 : R1R12

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Cleaning-in Place, CIP 1) To remove precipitated or denatured proteins and hydrophobic substances, wash the column with 5-10 bed volumes of 30% Isopropanol for 15-20 minutes of contact time followed by 10 bed volumes of ultrapure water washed