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Study procedures included a telephone pre-screening to check for eligibility according to the inclusion/exclusion criteria, a battery of online psychological and demographic questionnaires, a face-to-face assessment including a structured clinical interview and further clinical assessments, and a magnetic resonance imaging (MRI)/MRS session (see subsections below for details)

N Engl J Med 1993;328:22832

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The cleared lysate was then mixed with nickel-nitrilotriacetic acid (Ni-NTA)-agarose beads (QIAGEN, Hilden, Germany) or glutathione (GSH) Sepharose 4B beads (Cytiva, Marlborough, MA, USA), and incubated at 4 C for 1 h with rotation to collect tagged proteins