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Dying neurons labeled with Fluro-Jade B were present in the hippocampus and cortex of vehicle treated pups after HI

TMA-containing compound assay Trimethylamine-containing compounds were extracted from 50 l of plasma, 50 l of whole liver homogenate or from 100 l of whole brain homogenate (without the cerebellum and olfactory bulbs) by adding 10 volumes of ACN and spiking the samples with 5 l of IS mix containing 12 M d9-TMAO (CIL), 649 nM d9-TMA (CDN Isotopes), 6 M d3-L-carnitine (CIL), 329 nM d9-crotonobetaine (see Koeth et al., 2014) and 5 M d9-TML (Santa Cruz Biotechnology) at the beginning of the extraction

10.1099/00221287-112-2-315 [DOI] [PMC free article] [PubMed] [Google Scholar] 131

10.1038/nrc.2016.89 Nat

doi: 10.3389/fnut.2026.1775264 Received 25 December 2025 Revised 06 March 2026 Accepted 01 April 2026 Published 18 May 2026 Volume 13 - 2026 Edited by Eknath D
