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In the present study, we combine untargeted proteomics with an optimized tear fluid proteomics workflow, including in-strip protein digestion, which has previously identified over 3000 unique proteins in tear fluid 21

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At 20 mg in 3 mL (6.667 mg/mL), that is about 0.0375 mL, which reads as roughly 4 units on a U-100 syringe (125 mcg of each peptide)

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RF2 is monomeric both in solution and in the asymmetric unit of the crystal (Supplementary Fig