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doi: 10.1002/ibd.21048, 17

The membranes were first blocked with a 3% solution of BSA in TBST (tris-buffered saline with Tween) (20 mM Tris-HCl, pH 7.6, 150 mM NaCl, 0.1% Tween-20) at room temperature for 1 h

Furthermore, the repressed expression of several subunits of the NADH dehydrogenase respiratory complex I, one of the main ROS producers in mitochondria, renders neurofibromin-deficient cells more resistant to pro-oxidant drugs acting through complex I-mediated ROS increase

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