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These two cell types were then cocultured in a microfluidic device ( Figures 4C-F ) composed of two PDMS chambers separated by a porous (10 m) membrane

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Results from a commercial kit (purchased from Roche) measuring cell survival by detecting leakage of lactate dehydrogenase (LDH) were found to be misleading for this cell model Comparison of results obtained from the two different methods showed that the LDH detection did not correlate with the NR assay for all inducers tested and was not correlated with obvious morphological changes observed by light microscopy

A possible relationship between endocytosis, tolerance, and addiction risk is still being resolved (Berger and Whistler, 2010)