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Description
ROS have been implicated in a number of pathologic processes, including reperfusion injury, cancer, inflammatory disease, and aging

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Fumarate itself is a Michael acceptor, and the covalent attachment to protein cysteines corresponds to the post-translational modification known as succination [142] and fumarate has been recently demonstrated to yield a glutathione thioether in in vitro treated cells [142]
[DOI] [PMC free article] [PubMed] [Google Scholar] 163.Ko KS, Yang HP, Noureddin M, Iglesia-Ara A, Xia M, Wagner C, Luka Z, Mato JM, Lu SC

Thus, a GST 4QN fusion tag is recommended to prevent aberrant pulldown experiments
