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This is followed by a surge in the formation of reactive oxygen species (ROS), which cause oxidative bursts in the essential cellular components, including nucleic acids, lipids, and proteins [6]

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Some sources recommend gentle swirling, and that is acceptable, but the best approach is often just patience

However, we showed that the increase of NO resulted in positive regulation of p53, which potentiated the antioxidant defense response mediated by NFE2L2 through direct upregulation of Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1a) [44,45]