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Cytotoxicity assays, including MTT and SRB, showed significantly reduced toxicity for the PEGylated nanoparticle formulation compared to free tolcapone (cell viability exceeded 85% at 10, 25, and 50 M of the formula after 24 and 48 h of treatment)

1419) (Diagen, Ljubljana, Slovenia) dissolved in 0.9% NaCl was used in all experiments [1,2,3,4,5,6,7,8,9,10,11]

This holds particular promise for: Older adults facing chronic low-grade inflammation Immunocompromised individuals Age-related decline in vaccine efficacy Oncology patients recovering from chemo- or radiotherapy-induced immune depletion In the next section, we turn to the brain by examining Epitalons neuroprotective potential and early findings on cognitive health and neurodegeneration