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RAW 264.7 (ATCC TIB-71) mouse macrophages were cultured following the manufacturers instructions using DMEM supplemented with 10% FBS (non-heat inactivated) (ATCC, USA) and then were seeded at 1 10 4 cells/cm2

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The IVD tissue contains an autonomous oscillating peripheral clock, and evidence suggests that disruptions in these circadian rhythms can accelerate tissue aging and increase the risk of IDD

[8], [9] MOTS-c peptide has emerged as a potential factor of research interest in exceptional longevity, particularly in certain human populations such as the Japanese