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Finally, by adding it, you can be confident that the prepared solution has the correct balance and won't harm your cells in any way

Paw volume was calculated in l by subtracting the initial paw volume (basal) to the values measured at each time point (oedema)

[DOI] [PubMed] [Google Scholar] 76.Duffy S.L., Lagopoulos J., Cockayne N., Lewis S.J., Hickie I.B., Hermens D.F., Naismith S.L

doi: 10.1007/s10517-005-0359-2

Springer-Verlag: New York, 2014