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however, PDH activity was not detectable (Figure 3A)

This may influence multiple hallmarks of agingfrom oxidative stress and immune balance to circadian rhythm and sleep quality

We then evaluated histone acetylation on the OXTR promoter using the acetyl-histone H4 (K5, K8, K12, K16) antibody that recognizes histone H4 acetylated at lysines 5, 8, 12, or 16 and the acetyl-histone H3 (K9, K14, K18, K23, K27) antibody that recognizes histone H3 acetylated at lysines 9, 14, 18, 23 or 27 by ChIP analysis, and the results showed that there was no significant difference in either histone H3 or H4 acetylation (see Supplementary Figure 3b)