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To further investigate how the three missense mutations (I20B Q, L23B R and V24B N, Figure 2) led to a significantly increased GLP-1R ECD binding affinity, a comprehensive structural biophysical analysis [34] was conducted for the 10000 structural models of semaglutide mutant 524 built by Modeller [29]

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10.1371/JOURNAL.PONE.0029830 PLoS One 204 KuzumakiN.IkegamiD.ImaiS.NaritaM.TamuraR.YajimaM.et al (2010)

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