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For data analysis, CYP mRNA was quantified and normalized to the reference gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) using the DCt method

Accumulated free iron provides abundant substrate for this reaction, as the iron reacts with hydrogen peroxide to produce highly reactive ROS

Bertolini M, Zilio F, Rossi A, Kleditzsch P, Emelianov VE, Gilhar A, Keren A, Meyer KC, Wang E, Funk W, McElwee K, Paus R (2014) Abnormal interactions between perifollicular mast cells and CD8+ T-cells may contribute to the pathogenesis of alopecia areata

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