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Here is a detailed examination of these challenges and the corresponding solutions: Tag-Induced Protein Aggregation Challenge : One of the primary issues with GST-tagged proteins is that the GST tag can sometimes induce aggregation or interfere with the folding of the target protein

Discontinue use if gastrointestinal effects occur

M., Yatin M., Aulick T., Ain K

doi: 10.1155/2018/3029379 26 WangYZhaoFRijntjesEWuLWuQSuiJet al

Additional cytokines activate other components of the immune system, including cytotoxic T-cells, B-cells, and NK cells [9,10,95]