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Dilute cells to a density of 7.5 10 5 cells/mL in RPMI 1640 cell culture media (2 mM L-glutamine, 10% FBS)
PLoS ONE 8 , e79937 (2013)
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Supplementary Figures S57)
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The lack of VPS29 enrichment in either VPS35L proximity proteomes may reflect a technical caveat or arise from a restricted capacity for biotinylation due to the distinct mechanism for VPS29 association within the Retriever assembly 20,21,22
