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Van Lanen SG Enzymatic Strategies and Biocatalysts for Amide Bond Formation: Tricks of the Trade Outside of the Ribosome

[DOI] [PubMed] [Google Scholar] 204.Rivers-Auty J., Tapia V.S., White C.S., Daniels M.J.D., Drinkall S., Kennedy P.T., Spence H.G., Yu S., Green J.P., Hoyle C., et al

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Briefly, protein samples were heated at 88 C for the detection of ubiquitinated uS10 and otherwise at 95 C for 10 min, followed by centrifugation at 16,000 g for 10 min at room temperature before loading to gels

El-Hamouly, M., & Ibraheim, M