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After overnight expression at 15C and 250 rpm cells were collected by centrifugation, resuspended in BugBuster lysis buffer (Novagen) and incubated for 20 minutes at 20C

Plant Cell Environ 35: 454484 Queval G, Thominet D, Vanacker H, Miginiac-Maslow M, Gakire B, Noctor G (2009) H2O2-activated up-regulation of glutathione in Arabidopsis involves induction of genes encoding enzymes involved in cysteine synthesis in the chloroplast

A., Rudolph, D., Lan, Z
Chirani, A
Allosteric modulation of nicotinic and GABAA receptor subtypes differentially modify autism-like behaviors in the BTBR mouse model