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3 Proteins, gut microbiota, and depression 3.1 Association between protein intake and depression Adequate protein intake is essential for human growth, development, and health maintenance (12), and it is also associated with the prevalence of depression (2730)

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Total RNA extraction and RT-qPCR Total RNA was extracted from tissues and cultured cells using the RNAiso Plus reagent (Takara Bio, Tokyo, Japan) in accordance with the manufacturers protocol

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