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2024;67(3):488-503.[DOI] 147

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Mood Changes: B12 deficiency can affect mood, leading to symptoms such as irritability, depression, or anxiety

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Ongoing research and analytical verification are crucial to further understanding its role in peptide therapy and its potential applications in addressing various medical conditions

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Methods Cloning, expression, and purification of human CBS proteins The gene for human CBS (UniProt P35520) was cloned into pNIC-Bsa4 and pNIC-CH encoding for a TEV cleavable N-terminal and permanent C-terminal His-tag, respectively

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As both 5637 cells and our primary USCs are of basal cell origin, we seeded and cultured them on transwells for 23 weeks to compare cell differentiation phenotypes

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