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2024;67(3):488-503.[DOI] 147

Mood Changes: B12 deficiency can affect mood, leading to symptoms such as irritability, depression, or anxiety
Ongoing research and analytical verification are crucial to further understanding its role in peptide therapy and its potential applications in addressing various medical conditions

Methods Cloning, expression, and purification of human CBS proteins The gene for human CBS (UniProt P35520) was cloned into pNIC-Bsa4 and pNIC-CH encoding for a TEV cleavable N-terminal and permanent C-terminal His-tag, respectively

As both 5637 cells and our primary USCs are of basal cell origin, we seeded and cultured them on transwells for 23 weeks to compare cell differentiation phenotypes
