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Cell supernatants and lysates were plated on 7H11 agar medium (Hi Media, Santa Maria, CA, USA) enriched with ADC (GEMINI, USA) and incubated at 37C for three weeks to evaluate mycobacterial growth or survival under treatment conditions by counting the colony-forming units (CFUs)

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After six-months of supplements, improvements in subjective symptoms and thyroid function parameters were assessed

doi: 10.3389/fimmu.2023.1186383 Received 14 March 2023 Accepted 19 May 2023 Published 05 June 2023 Volume 14 - 2023 Edited by Steven Fiering, Dartmouth College, United States Reviewed by Paramita Chakraborty, Medical University of South Carolina, United States

MOTs-C If a patient complains that they are not losing weight or they have plateaued, MOTS-c can be given as a weekly injection to boost weight loss