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L-Glutamine induces a rapid tyrosine phosphorylation of EGF receptor (77, 78)

Pharmacological PKC, Trk, caspase, autophagy, translation, and necroptosis inhibitors Stocks of PKC inhibitor G6976 (10 mm, Tocris 2253), Trk receptor inhibitor K252a (200 m, Calbiochem #420298), pan-caspase inhibitors Boc-D-fmk (10 mm, Abcam ab142036), zVAD-fmk (20 mm, R&D Systems FMK001), and necroptosis inhibitor necrostatin-1 (NEC-1

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Simultaneously, the mechanical properties of the ECM change significantly: the shear stiffness of the human nucleus pulposus increases from 12.5 kPa in Pfirrmann grade 116.5 kPa in grade V (Walter et al., 2017), collagen fibers thicken and become disorganized (Leung et al., 2014), its aggrecan synthesis decreases while degradation increases (Wuertz et al., 2007) (Table 1)

Pellegrino R, Paganelli R, Di Iorio A, Bandinelli S, Moretti A, Iolascon G, et al