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After washing three times with TBST (Tris-buffered saline supplemented with 0.1% Tween-20), the samples were incubated for 1 hour at room temperature with a secondary antibody

Pharmacological Reviews, 60, 243260

ChristensenJEl-GebaliSNatoliMSengstagTDelorenziMBentzSet al

Then have a colleague check it
Both patients were desensitized in hospital conditions according to the same scheme (Table 4)
