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Incubations were carried out at 37C for the time stated in each case in (a) distilled water or phosphate buffer saline (PBS) and then lyophilized
After washing twice with UA as above, 100 L of 50 mM AmBic (50 mM NH 4 HCO 3 in water) was added to the filter unit and the filter was centrifuged at 14,000 g for 10 min
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Clinimetrics: physiotherapy evidence database (PEDro) scale
Ensure quality sleep and stress management to optimize recovery and healing responses
