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Interestingly, GLS1-knockout CD4(+) T cells exhibited an increase in the proportion of T helper 1 (TH1) cells, which showed excessive effector function and an increase in exhaustion signatures, but in cytotoxic CD8+ T cells in culture with limited glutamine or treated with a glutamine metabolism inhibitor prevented exhaustion, promoted memory-like T cell differentiation and tumor elimination in vivo [89, 90]

To assess the variation in expression between replicates and samples of different time points, we conducted Principal Component Analysis (PCA) and a heatmap using correlation analysis

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