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Next, membranes were blocked with 5% BSA in Tris-buffered saline containing 0.1% (v/v) Tween 20 (TBST) for 1 h at room temperature and probed with primary antibodies against corresponding antigens overnight at 4 C

A., & Liu, G

When handled correctly, the treatment will not compromise its safety

Vitamin E and glutathione work through different but complementary antioxidant mechanisms, vitamin E primarily protecting cell membranes whilst glutathione works in the water-soluble portions of cells
