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[DOI] [PMC free article] [PubMed] [Google Scholar] 41.Hulsmann W.C., Peschechera A., Serafini F., Ferrari L.E

GHK-Cu upregulates these enzymes plus glutathione reductase (regenerating reduced glutathione) and glucose-6-phosphate dehydrogenase (providing NADPH for glutathione reduction)

In addition, in order to account for the blood sampling at convenience we adjusted for the time since last meal in the analyses

Mass spectrometry analysis of HNEylation site Following the steps described in T-REX in HEK293T cells, cell lysis (3 freezethaw) was performed in 500 l (50 mM HEPES (pH 7.6), 100 mM NaCl, 1% Nonidet P-40, 10 mM imidazole, 5 mM -mercaptoethanol and Roche protease inhibitor)

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