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Thus, amphoteric molecules are located in narrow bands where the pI coincides with the pH

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Protein quantification was performed by densitometry analysis of whole iWAT or iWAT samples enriched in mitochondria separated on 12.5% SDSPAGE gels and using a low-molecular-weight protein calibration kit (Amersham, GE Healthcare)

Additionally, a study using the extract from amla leaves (200400 mg/kg BW) indicated a similar protective effect in diabetic mice by reducing inducing the activity of GSH, GPx, SOD, and CAT activity and also reducing lipid peroxidation [38]