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Under basal condition, Nrf2 interacts with two molecules of Keap1 through its Neh2 ETGE and DLG motifs to activate Cullin 3 (Cul3)based E3 ligase complexmediated Nrf2 ubiquitination reaction.7 Once Nrf2 is ubiquitinated, it will be rapidly degraded by 26S proteasome and maintained at a very low level in the cytoplasm.8 On exposure of cells to oxidative stress or chemopreventive compounds, the cysteine residues of Keap1 are modified and the conformation changes, resulting in the detachment of Nrf2 DLG motifs from Keap1, disrupting the ubiquitination and degradation of Nrf2

5.9.5 Intracellular cytokine staining Splenocytes were prepared and stimulated in 96-well U-bottom plates with 1 million cells/well

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The GSH depletion caused by MEN was also prevented by MEL, counteracting the oxidative stress and apoptosis provoked by the quinone