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Counting ovarian structures Using a Leica CM1900 Cryostat and a standard H&E staining protocol 38 , histological ovarian sections were made available for quantifying ovarian follicles, corpora lutea and cysts using a modified validated protocol combining systemic random sampling and the optical dissector method on Image J NIH software system (National Institutes of Health, Bethesda, MD, USA) 39,40