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There are a number of reasons that could attribute to this, mainly the low concentration of protein in the cell lysate used for each reaction

Glutathione-triggered mitigation in salt induced alterations in plasmalemma of onion epidermal cells

Upon irradiation with visible light (410 nm), PPIX generates reactive oxygen species (ROS) 24 , which oxidizes Met to Met sulfoxide (MetO), thereby rebalancing noncovalent forces driving a morphological transition from nanofibers (NFs) to spherical nanoparticles (NPs)

J Thorac Cardiovasc Surg 154(4):1349-1351, 2017

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