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Some medications may require single-dose sterile water or other specific diluents

After reading about your B12 studies, I realize my doctor has been giving me the cheap cyanide B12

In practice, this likely contributed a small amount of oxygen (from the wet paper towel), as EcN did typically form a biofilm ring at the air-liquid interface under this condition, which was absent for larger-volume biofilm cultures in culture tubes that were incubated strictly anaerobically without humidification

The placebo control arm received 7.2 g of isomaltulose with the same way of administration

If more detailed analysis is needed, laboratory water testing may be recommended to detect contaminants like bacteria, nitrates, or heavy metals, which may involve an additional cost