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WHAT IS B12

Then, the cells were lysed on ice in 800 l of lysis buffer 1, which was composed of 7 M urea (J.T.Baker), 30 mM tris(hydroxymethyl)aminomethane (Sigma-Aldrich), 2 M thiourea (Sigma-Aldrich), 0.1% Triton X-100 (Sigma-Aldrich), protease inhibitors (EDTA free cocktail tablet, Roche Diagnostics, added according to the manufacturers instructions) and phosphatase inhibitors [1 mM sodium orthovanadate (Sigma-Aldrich), 9.5 mM sodium fluoride (J.T.Baker) and 1 M okadaic acid (Sigma-Aldrich)]

Ethics statement The animal study was approved by the Institutional Animal Care and Use Committee at Cairo University, Cairo, Egypt (Vet CU13102024983)

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