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The mycobacterial iron-dependent regulator (IdeR) is crucial to the maintenance of iron homeostasis in Mtb as experimentally-induced lack of IdeR results in an accumulation of iron, leading to oxidative damage and subsequent death of the mycobacterium, thus highlighting the importance of exochelins and IdeR as Mtb -survival mechanisms (147)
PMID 12006124
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To test these hypotheses, we used quantitative PCR assays to monitor the expression of carefully selected genes 14 , HSP70, encoding heat shock protein 70, an indicator of cell stress 15,16 , tumour protein P53 ( P53 ), a central transcriptional factor activated by stressors such as UV and involved in cell cycle arrest, DNA repair and apoptosis 17,18 and KIN17 ( KIN ), a cell cycle control protein up-regulated by UV 19,20 and constructed linear mixed effect models 14,21
[DOI] [PubMed] [Google Scholar] 21.Sotiropoulos A, Ohanna M, Kedzia C, Menon RK, Kopchick JJ, Kelly PA, Pende M
