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For the amplicon PCR, a 25 L reaction containing cDNA, 0.5 L of Platinum SuperFi II DNA Polymerase, 1 SuperFi II Buffer, and 500 nM forward and reverse primers was first heated to 98 C for 30 s, followed by cycles of 98 C for 10 s, 64 C for 10 s, and 72 C for 20 s

[DOI] [PMC free article] [PubMed] [Google Scholar] 87.Brutsch S.H., Wang C.C., Li L., Stender H., Neziroglu N., Richter C., Kuhn H., Borchert A

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2012;3(5):e15

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