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The frequency of immune cell subsets was assessed by staining 1 x 10 6 nucleated cells with CD45 (KO), CD56 (PC7), CD138 (APC) (both Beckman Coulter), CD14 (PerCP), CD19 (APC-H7), CD3 (V450) and CD16 (PE) (all BD Biosciences)

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Older adults Vitamin B12 deficiency affects older women over the age of 50 by putting them at risk for various conditions that lead to a decrease in stomach acid production.When levels of stomach acid are low, a particular bacteria that uses B12 begins to grow, thus reducing the amount of B12 that is available to the body