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The cells were washed twice with PBS and then fixed with 4% paraformaldehyde for 20 min

ASS1 expression is downregulated in CHOL, GBM, KICH, KIRC, KIRP, and LIHC tumor categories, suggesting the feasibility of arginine depletion therapy

In addition, PFD can also inhibit differentiation of myofibroblasts by regulating PDFG, a fibroblast mitogen receptor, but the specific mechanism is still unclear (Ruwanpura et al., 2020)

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