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The inoculated MRS broth tubes were incubated at 37 C for 24 h

Studies should be designed to verify this and optimize dosing because such intervention would be high yield, low risk, and affordable

However, CS flies showed dampened locomotor activity on day 2 of constant CL and completely arrhythmic locomotor activity beginning on day 3 of constant CL condition

The statistical programming language R version 4.0.0 was used [15], with the R packages lme4 version 1.127.1 and lmerTest version 3.13 to compose the LMMs [16]

10.1371/journal.pone.0138065 167 SensiF.D'AngeloE.D'AroncoS.MolinaroR.AgostiniM
