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Gunn, the cancer centers chief operating officer from 1987 to 2015, said board members felt gems of research were lying fallow and nobody was kind of pushing it, to commercialize it. By the time Varmus left to direct the National Cancer Institute in 2010, Memorial Sloan Ketterings board was looking for a leader who was as comfortable in a corporate boardroom as in the lab, according to several longtime current and former cancer center employees

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To delete FBXL5 in DC2.4 cells, FBXL5-targeting lentiCRISPR v2 plasmids were co-transfected with the packaging plasmids, pLenti-P2A and pLenti-P2B (LV003