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On the basis of the high correlation obtained on comparing the results of BChE analysis of 49 normal human sera by the Michels pH method with different other methods including the kinetic Ellman method, MacQueen et al

10.1038/ncomms11253 365 WilliamsK

After harvesting the cells by centrifugation at 4,000 g for 30 min, the cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 8.0, 300 mM NaCl, 10 mM imidazole) and lysed using a sonication for 1 h with a 10-s pulse mode (10 s on and 10 s off) on ice

their presence is suggested by Blastp reported partial sequences of cytochrome cd 1 nitrite reductase in several Bacillus

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